Lab Sequencing Services and Techniques
Sequencing processes offered are extremely accommodating and multi-faceted. DNA sequencing services are full range, high-throughput, and have solutions facilitated by personal strategic choices. Sequencing projects are a top priority, and handled with conscientiousness and professionalism. Techniques are performed with very modern equipment and practices, and requires certain details in order to guarantee the best quality sequencing.
Templates and Specifics
Templates to organize data and information is available here. DNA should be premixed with primer, with the previous link detailing the concentrated amounts for specific samples. There are recommended concentrations of DNA to be sent, with primers requiring a certain guidelines such as avoiding palindromes and requiring designs from accurate sequence data.
Samples that should not be sent are ones with RNA, traces of ethanol, traces of phenol, or high concentrations of salt. Salt and protein cannot be used due to the capillary based DNA analyzer in the lab. Their presence lowers signals due to the possibility of negative ions in salts being directly injected into capillary arrays.
Preventing Poor Results
Failure can occur for multiple reasons. Conditions must acclimate the primer being the correct °C, the template DNA cannot be of poor quality (insufficient primer concentration, too little DNA causing no signal, residual salt and ethanol). Be sure to follow guidelines with the lab and be organized and precise when the DNA is getting the primer concentrations. Ensure that samples do not accidentally contain two or more PCR materials, and that PCR fragments are properly gel purified during purification sequences.
Getting Your Sequencing Done
Procedures are available online to order. Contact is always available by phone for email for questions and suggestions. Once sequence results are finished, they can be accessed online and include the densitometric scan and the sequence data.